Cellular Basis of Life
Chapter 2
Cell
Cell was first discovered by Rober Hook in 1665. Cell is the structural and functional unit of a living thing. All living things are made of microscopic units i.e. cells.
Cell Theory
This theory was put forwarded by Schwann (1838 to 1839) and later on by Virchow (1858). According to this theory:
Types of Cell
Basically cells are of two types namely:
"The cells in which true or complete nucleus is not present, instead, genetic material is found scattered in the cytoplasm is called Prokaryotes."
In such cells, DNA is found in the form of fragments or chains not enveloped or enclosed by a nuclear membrane. Such type of Nucleus is called Incapient Nucleus. Other membrane bounded organelles like Colgibodies and Mitochondria are also absent in these cells. Examples of Prokaryotes are Virus, Bacteria, and Blue Green Algae.
"The cells having complete nucleus bounded with a nucleur membrane is called Eukaryotes."
These cells also have got other membrane-bounded organelles like mitochondria, colgibodies etc. Examples of Eukaryotes are Algae, Fungi and other plants and animals.
Structure of Cell
The basic structure of plant and animal cell is almost the same except certain differences. The basic structure of a cell is composed of the following components.
However in plants, a rigid "Cell wall" is present outside the cell membrane or plasma membrane.
Cell Wall
It is present in Bacteria and plant cells. The cell wall lies outside the cell membrane.
Chemically, it is made up of dead substances like cellulose and Pectine secreted by the protoplasm. Sometimes another substance Lignin is also present.
Functionally, cell wall serves to protect the protoplasm and to give a definite and rigid shape to the cell.
Structurally, cell wall is many-layered thick. Finally, Middle lamella is laid down between the two cells; Secondly, Primary cell wall is deposited between Middle lamella and the cell membranes of both the cells. Finally, Secondary layer of cell wall is deposited on the inner surface of Primary Cell Wall. The fibres of each layer are laid at different angles to provide maximum tensile strength to the cell wall.
Cell wall is also has got pores and is permeable in nature.
It is also called Plasma membrane. In animal cell, it is the outermost boundary whereas in plant cell, it lines the cell wall internally. It surrounds the cytoplasm in both types of cells.
Chemically, it is made up of Proteins and Lipids. Structurally, it is made up of three distinct layers, an outer and inner layer of proteins and a sandwiched layer of lipid. This three-layered structure was proposed by Dawson and Donelli.
Electron Microscopic Structure
The sandwiched layer of lipid is bimolecular whereas the protein layers are monomolecular. Lipid layer forms the gross structure of membrane. Lipids of membrane are of four types namely Phospholipid, Glycolipid, Cholesterol and Sterol. Out of these, phospholipid is the most important structural component. Each molecule of phospholipid has got two ends i.e.
Hydrophilic end is made up of Phosphate while hydrophobic end is composed of fatty acid joined with the Glycolipid. The two layers of hydrophobic ends face each other while phosphate ends faces proteins layers. Cholesterol serves to join the phosphate molecules.
Fluid Mosaic Bilayer Model
It was observed that Protein molecules are located in the outer and inner surface and are called "Extrinsic Molecules" while other penetrates the membrane called "Intrinsic Molecules". So it can be said that bimolecular layer of lipid serves as a sea in which protein molecules float as icebergs. This hypothesis was suggested by Singer and Nicholson in 1970 who named it "Fluid Mosaic Bilayer Model."
Functions of Cell Membrane
Different Types of Membrane Permeabilities
(a) Osmosis
"Movement of solvent molecules from their higher concentration towards their lower concentration is called Osmosis."
Or
"To maintain an equilibrium between interacellular fluid and interstitial fluid through cell membrane is called Osmosis."
(b) Diffusion
"Movement of a substance from its higher quantity towards its lower quantity is called Diffusion."
Or
"Ability of a substance to spread in all directions is called Diffusion."
(c) Active Transport
"The phenomenon in which a substance is tansported from its lower quantity towards its higher quantity through cell membrane with the expenditure of energy is called active transport.
(d) Phagocytosis
"Engulfment of solid particles by the cell membrane is called Phagocytosis."
(e) Pinocytosis
"Uptake of droplets or fluid in bulk by the cell membrane is called Pinocytosis."
The living ground material of the cell filling the space between the cell membrane and nucleus is called cytoplasm. It is fluid to semi solid in consistency. Living cytoplasm shows streaming movement along cell membrane called Cyclosis. To perform different functions, cytoplasm contains different kinds of organelles. These organelles are as follows;
It is a complex network of channels extending from cell membrane to nuclear membrane. These channels are made up of lipoproteins.
Endoplasmic reticulum is of two types:
Smooth endoplasmic reticulum is not coated with ribosomes and is generally found in Adipose tissue, muscle cells and glycogen storage cells. Generally both types of Endoplasmic Reticulum are present in most of the cells.
Rough or Granular Endoplasmic Reticulum is coated with ribosomes and is generally found in protein synthesising cells such as Pancreatic, hepatic and other glandular cells.
Endoplasmic reticulum forms the structural frame work of the cell, it provides space for the attachement of Ribosomes and increases the surface area to enhance different chemical reactions by means of enzymes attached with the membranes of their channels. The channels serve as pathway for the transport of various substances from one place or organelle to another.
These are tiny organelles abundantly found in all cell types. In Prokaryotes they are freely dispersed in the cytoplasm while in Eukaryotes they are found freely as well as bounded with the membranes of endoplasmic reticulum. Some of the ribosomes form free floating chains called "Polysomes".
Each Ribosome contains Ribo Nucleic Acid and produce different types of Proteins required by the cell under guidance of Messenger-RNA of the Nucleus. In short "Ribosome is the protein factory of the cell."
Ribosomes are produced in the Nucleolus. Structurally they are spheroidal in shape having two unequal units, the larger sub-unit is adherent with the Endoplasmic Reticulum whereas smaller subunit forms a cap on the large sub-unit.
They are only visible under low frequency imaging in electron microscope. These are straight skeletal structures made up of a contractile protein called Tubulin. Microtubules are concerned with locomotary organs like Cilia and Flagellum etc. They also form spindle fibres for chromosomal movement during cell division.
These are small rod shaped, vesicular or thread like structures scattered through out the cytoplasm. They are variable in number and present in animal and plant cells.
Each mitochondria is 2.5nm long and about 1.0nm in diameter. It is bounded with two delicate membranes. The inner membrane is folded forming partitions called "Cristae". Inside the mitochondria, Matrix is filled, containing enzymes for catabolism of food substance. Functionally, mitochondria are regarded as "Power house" of the cell by performing Respiration.
This mysterious structure was discovered by an Italian scientist Colgi in 1898. Under Electron microscope, it appears as a cluster of loose sac, especially in "Glandular Cells". Colgibody or apparatus synthesizes enzymes, Lipids and substance required for membrane formation.
It is found in animal cells and certain lower plant cells. Each centrosome appears during cell division near the nucleus. It is composed of two smaller organelles called "Centroids", Each one is composed of hollow tubules. During cell division both centroides move in opposite direction, between them spindle fibres are produced for the distribution and movement of chromosomes.
It is a characteristic cytoplasmic organelle of plants, totally absent in animals. It is also a membrane-bounded organelle serving as a chemical synthesizer, photo-battery and storage body.
Each plastid is a plate like structure or a spindle shape body containing different pigments, Plastids are classified according to their pigment into three types, i.e.
They contain chlorophyll, a green pigment capable to convert sunlight into electrochemical energy. Chloroplasts are found in green parts of the plants, making them capable to manufacture food by means of photosynthesis.
These are colourless plastids found in unexposed parts of plants such as roots and underground stems. These plastids convert sugar into starch for storage purpose.
These are coloured plastids found in coloured parts of plant such as flower, fruits and certain leaves. Different pigments present in chromoplasts are b Carotene (orange), Xanthophyll (Yello), Anthocyanin (blue) etc.
These are enzyme filled organelles originated by the Colgibody. These enzymes are kept separated from cytoplasm by means of a membrane called tonoplast. Lysosomes are generally present in animal cells only.
Lysosomes are used in the digestion of solid particles (taken up by the cells by means of Phogocytosis) or out-lived other cellular organelles.
Vacuoles are bubble like cavities filled with different substances surrounded by "Tonoplasts". Vacuoles are large and conspicuous in plant cells filled with water, reserved food, enzymes and minerals.
In animal cells, especially lower animals or Protozoans vacuoles are of two types i.e.
Robert Brown in 1831 discovered a central tiny ball or spherical structure in the centre of cell, he named it nucleus.
Later on it was discovered that nucleus is the most important part of the cell and controls all its vital activities and division i.e. Reproduction.
Nucleus is generally single, circular and bounded with a membrane called "Nuclear Membrane". It has got pores called "Nucleopore" through which the cytplasm communicates with the ground substance of nucleus called "Nucleoplasm" or "Karyolymph". It is made up of proteins, in this dense liquid substances are scattered a number of delicate thread like structures called "Chromatin Network". This mesh of chromatin is made up of DNA and contains the hereditary information.
The DNA strands or Chromatin filaments condense to form assorted thicker threads called chromosomes during cell division.
Each specie contains a fixed number or definite number of chromosomes generally denoted as "2n" and called diploid number, eg 46 in humans, 80 in sugar cane.
Within the nucleus one or two spherical bodies are also found known as "Nucleolus". Each nucleolus contains RNA and serves to produce Ribosomes.
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